Maternal Sevoflurane Exposure Causes Abnormal Development of Fetal Prefrontal Cortex and Induces Cognitive Dysfunction in Offspring

Figure 2

Maternal sevoflurane exposure decreased the production of deep-layer immature neurons. (a) Schematic diagram of the timing of sevoflurane exposure and sacrifice to assess the differentiation in the fetal PFC. (b, c) Tbr1 (green) and Satb2 (red) immunofluorescence, combined with DAPI staining (blue) in the cortical plate at G18.5. Scale bars, 20 μm. (d) Quantification of the Tbr1⁺ and Satb2⁺ cells of the control and sevoflurane groups. (e, f) NeuN (red) immunofluorescence and DAPI staining (blue) in the cortical plate at G18.5. Scale bars, 20 μm. (g) Quantification of the NeuN⁺ cells of the control and sevoflurane groups. (h, i) GFAP (red) immunofluorescence and DAPI staining (blue) in the cortical plate at G18.5. Scale bars, 20 μm. (j) Quantification of the GFAP⁺ cells of the control and sevoflurane groups. Data are expressed as the mean ± SEM. .

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