Maternal Sevoflurane Exposure Causes Abnormal Development of Fetal Prefrontal Cortex and Induces Cognitive Dysfunction in Offspring

Figure 6

Maternal sevoflurane exposure did not influence the duration of G1-, M-, and G2-phases of neural progenitors in the fetal PFC. (a) Schematic diagram of the timing of sevoflurane exposure and sacrifice to assess the expression of Ccnd1 and PH3. (b, c) Ccnd1 (green) immunofluorescence and DAPI staining (blue) in the cortical plate at G15.5. Scale bars, 20 μm. (d) Quantification of the Ccnd1⁺ cells of the control and sevoflurane groups. (e) PH3 (green) immunofluorescence and DAPI staining (blue) in the cortical plate at G15.5. PH3-positive cells were counted in radially arranged 200 mm-wide boxes, as illustrated. Scale bars, 20 μm. (f) Quantification of the PH3⁺ cells of the control and sevoflurane groups. Data are expressed as the mean ± SEM.

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