Research Article
Y-box Protein-1 Regulates the Expression of Collagen I in Hepatic Progenitor Cells via PDGFR-β/ERK/p90RSK Signalling
Table 3
Antibodies used in Western blot (WB) analysis and immunofluorescence (IF).
| | Antibody | Dilution | Supplier | Product ID |
| | YB-1 | 1 : 10,000 (WB),
1 : 1000 (IF) | Abcam | ab76149 | | p-YB-1 (ser102) | 1 : 2000 (WB) | CST | 2900 | | AFP | 1 : 500 (IF) | Abcam | ab46799 | | Albumin | 1 : 500 (IF) | Bethyl | A90-134A | | CK19 | 1 : 500 (IF) | Abnova | MAB12076 | | CD133 | 1 : 100 (IF) | eBioscience | 14-1331-80 | | CD90/Thy1 | 1 : 500 (IF) | Novus | NB100-65543 | | α-SMA | 1 : 500 (IF) | Abcam | ab7817 | | Collagen I | 1 : 1000 (WB),
1 : 200(IF) | Abcam | 6308 | | PDGF-β | 1 : 2000 (WB) | Abcam | ab178409 | | PDGFR-β | 1 : 2000 (WB) | Abcam | ab32570 | | p-Stat3 | 1 : 1000 (WB) | CST | 9145 | | ERK1/2 | 1 : 1000 (WB) | CST | 4695 | | p90 RSK (ser380) | 1 : 1000 (WB) | CST | 9341 | | PI3K p85 | 1 : 1000 (WB) | R&D | MAB2998 | | GAPDH | 1 : 10,000 (WB) | Abcam | ab8245 |
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