|
| Gene | Primer sequence
(5–3) | Annealing temperature (°C) | Product size (bp) |
|
| GAPDH | Forward: GTGGTCTCCTCTGACTTCAACA
Reverse: CTCTTCCTCTTGTGCTCTTGCT | 62 | 210 |
| OCT4 | Forward: GACAACAATGAGAACCTTCAGGAGA
Reverse: TTCTGGCGCCGGTTACAGAACCA | 62 | 218 |
| SOX2 | Forward: AACCAAGACGCTCATGAAGAAG
Reverse: GCGAGTAGGACATGCTGTAGGT | 62 | 341 |
| cMYC | Forward: TCGGATTCTCTGCTCTCCTC
Reverse: CGCCTCTTGACATTCTCCTC | 62 | 413 |
| KLF4 | Forward: ATTCTCTCCAATTCGCTGACCC
Reverse: TTCAGCACGAACTTGCCCAT | 62 | 376 |
| NANOG | Forward: ATAGCAATGGTGTGACGCAG
Reverse: GATTGTTCCAGGATTGGGTG | 62 | 219 |
| P53 | Forward: TCGACATAGTGTGGTGGTGC
Reverse: TTGGACTTCAGGTGGCTGGA | 58 | 480 |
| LIN28 | Forward: GCTCCGTGTCCAACCAGCAG
Reverse: TTTCCTTTTGGCCGCCTCTC | 58 | 376 |
| P16 | Forward: CGAATAGTTACGGTCGGAGG
Reverse: GCATGGTTACTGCCTCTGGT | 62 | 309 |
| DLX5 | Forward: ACCATCCGTCTCAGGAATCG
Reverse: ACCTTCTCTGTAATGCGGCC | 60 | 384 |
| RUNX2 | Forward: TTGCAGCCATAAGAGGGTAG
Reverse: GTCACTTTCTTGGAGCAGGA | 58 | 470 |
| PPARG | Forward: TCTCTCCGTAATGGAAGACC
Reverse: GCATTATGAGACATCCCCAC | 55 | 474 |
| C/EBPA | Forward: CCAAGAAGTCGGTGGACAAGAA
Reverse: TCATTGTCACTGGTCAGCTCCA | 62 | 145 |
| BMP7 | Forward: CCAACGTCATCCTGAAGAAATAC
Reverse: GCTTGTAGGATCTTGTTCATTGG | 60 | 271 |
| SOX9 | Forward: GGTTGTTGGAGCTTTCCTCA
Reverse: TAGCCTCCCTCACTCCAAGA | 61 | 400 |
| P21 | Forward: GCGATGGAACTTCGACTTTG
Reverse: CGTTTTCGACCCTGAGAGAGTC | 60 | 285 |
| OSTEOCALCIN | Forward: CGCAGCCACCGAGACACCAT
Reverse: GGGCAAGGGCAAGGGGAAGA | 62 | 405 |
| OSTEOPONTIN | Forward: GAGACCCTTCCAAGTAAGTCCA
Reverse: GATGTCCTCGTCTGTAGCATCA | 62 | 354 |
| COMP | Forward: GAAGAACGACGACCAAAAGGAC
Reverse: GTCACAAGCATCTCCCACAAAG | 62 | 232 |
| TYPE I COLLAGEN | Forward: CACAGAGGTTTCAGTGGTTTGG
Reverse: GCACCAGTAGCACCATCATTTC | 62 | 191 |
| AP2 | Forward: AAGAAGTAGGAGTGGGCTTTGC
Reverse: CCACCACCAGTTTATCATCCTC | 62 | 381 |
| LPL | Forward: AGAGAGGACTTGGAGATGTGGA
Reverse: GGAAGACTTTGTAGGGCATCTG | 62 | 264 |
|
