Epigenetic Manipulation Facilitates the Generation of Skeletal Muscle Cells from Pluripotent Stem Cells

Figure 3

H3K27me3 demethylation in hESCs by forced expression of JMJD3c. (a) Immunostaining analysis showing the H3K27me3 demethylation in whole nuclei of hESCs by JMJD3c overexpression (arrows). (b) ChIP-sequencing analysis showing the significant reduction of H3K27me3 at the PAX7 gene in hESCs by JMJD3c overexpression. H3K4me3 enrichment remains at the PAX7 gene after JMJD3c overexpression.

(a)

(b)