NURR1 Downregulation Favors Osteoblastic Differentiation of MSCs

Figure 3

Effect of NURR1 downregulation on osteoblast markers. (a)-(b) qPCR performed on si-NURR1 or CTR cells showed that NURR1 downregulation significantly increased the expression of the two osteoblast markers ALP (8 days) (a) and Col1 (6–8 days) (b). Expression was normalized to GAPDH. compared to CTR. (c) Immunoblotting confirmed that the expression of Col1 protein increased in NURR1 silenced cells relative to CTR cells (). Each graph represents means ± SE of 3 independent donors. Statistics: unpaired Student’s t-test.

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