In Vivo Tracking of Chemokine Receptor CXCR4-Engineered Mesenchymal Stem Cell Migration by Optical Molecular Imaging

Figure 5

In vivo migration and localization of transplanted MSC-CXCR4/Fluc2 in the tumors. (a) Rluc activity of MDAMB231/Rluc tumor xenografts. (b) Fluc activity of migrated MSC-CXCR4/Fluc2 and MSC/Fluc2 in breast cancer xenografts. (c) Quantitative data of Fluc activity from the lungs. (d) Quantitative data of Fluc activity from tumor. (e) Ex vivo Fluc imaging of migrated MSC-CXCR4/Fluc2 and MSC/Fluc2. (f) Immunohistochemistry analysis of GFP for MSC/Fluc2 and MSC-CXCR4/Fluc cells in MDAM231/Rluc tumor. Data were analyzed from 3 mice with the means ± standard deviation (SD), and the value <0.05 was considered as statistically significant by Student t-test.

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