In Vivo Articular Cartilage Regeneration Using Human Dental Pulp Stem Cells Cultured in an Alginate Scaffold: A Preliminary Study

Figure 2

Rabbit primary articular chondrocytes were isolated and cultured in proliferation (a–d) or differentiation culture medium (e–h) for up to 6 weeks. F-Actin expression (a, e) was analyzed by microscopy fluorescence using rhodamine-conjugated phalloidin, and collagen II (COLII; (b, f)), aggrecan (ACAN; (c, g)), and collagen I (COLI; (d, h)) expression were analyzed by immunofluorescence. In all panels, cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI). Microphotographs are representative fields of three independent experiments.

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