Capacity of Human Dental Follicle Cells to Differentiate into Neural Cells In Vitro

Figure 5

Immunofluorescence staining for neural markers in hDFCs following two-step neuronal induction. (a–d) Nestin staining. (e–h) β-III-Tubulin staining. (i–l) S100β staining. (a, b, e, f, i, j) A neurosphere cultured in NDM on day 0. (c, g, k) The cells in the neurosphere cultured in NDM on day 3 grew out from the sphere. (d, h, l) The cells in the neurosphere cultured in NDM on day 7 grew out from the sphere. Scale bars (a, e, i) = 100 μm and (b, c, d, f, g, h, j, k, l) = 50 μm. Arrows indicate cells that were strongly positive for the marker and that showed neural-like morphology with branched dendrites and refractile cell bodies.

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