Neuron-Specific Fluorescence Reporter-Based Live Cell Tracing for Transdifferentiation of Mesenchymal Stem Cells into Neurons by Chemical Compound

Figure 3

Neuronal differentiation of MSCs after treatment of NHPDQC. (a) Reverse transcription polymerase chain reaction (RT-PCR) results (left column: DMSO-treated group, right column: NHPDQC treatment group). Expression of the neuronal markers Tuj1 and NSE was increased in the MSCs at 48 h after treatment with NHPDQC. The RT-PCR results revealed that the expression of the presynaptic vesicle protein, synaptophysin, was increased slightly after 48 h of treatment with NHPDQC. Glial marker GFAP did not increase in both of undifferentiated and differentiated MSCs. (b) Immunofluorescence staining was performed in the DMSO-treated cell group and the group treated with NHPDQC. The results showed that Tuj1 expression was increased in the NHPDQC-treated group within 48 h. Green color: β-tubulin (upper panel), Tuj1 (lower panel); blue color: DAPI.

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