Neuron-Specific Fluorescence Reporter-Based Live Cell Tracing for Transdifferentiation of Mesenchymal Stem Cells into Neurons by Chemical Compound

Figure 4

Enhanced fluorescence signals after neuronal differentiation of pTα1-DsRed2-transfected MSCs by NHPDQC. (a) Confocal microscopic data showed that Tα1 promoter-regulated RFP reporter activity was increased in the NHPDQC-treated group within 48 h (blue color: DAPI; red color: pTα1-DsRed2). (b) Two days after treatment of NHPDQC, length of neurite outgrowth was measured from confocal microscope images. value <0.05. (c) Total fluorescence activity for the expression of neuronal markers was analyzed in whole cell population by TissueFAXS2.0.

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