Extracellular Vesicles from Adipose-Derived Mesenchymal Stem/Stromal Cells Accelerate Migration and Activate AKT Pathway in Human Keratinocytes and Fibroblasts Independently of miR-205 Activity

Figure 3

Extracellular vesicles derived from mesenchymal stem/stromal cells induce proliferation of fibroblasts and keratinocytes. (a) Growth curves comparing proliferation of fibroblasts (left graph) and keratinocytes (right graph) cultured in medium (DMEM or KSFM depending on cell type) in the absence (medium) or presence of extracellular vesicles (medium + EVs) derived from mesenchymal stem/stromal cells (MSCs). (b) A representative Western blot gel illustrating bands for total and phosphorylated histone H3 (p-histone H3). (c) Densitometry analysis of the p-H3/total H3 ratio. Incubation with MSC EVs was associated with higher relative levels of phosphorylated (activated) histone H3 in both fibroblasts and keratinocytes. Data are presented as the mean ± standard deviation of three biological independent experiments. Statistical significance of differences is indicated as follows: ; .

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