Gap Junctions Are Involved in the Rescue of CFTR-Dependent Chloride Efflux by Amniotic Mesenchymal Stem Cells in Coculture with Cystic Fibrosis CFBE41o- Cells

Figure 5

Effect of Cx43 siRNA on transepithelial resistance and cell viability. (a) Trans-epithelial resistance (TER; ohm cm²) was measured after 6 days from the transfection with either siRNA against Cx43 or scrambled siRNA. Untreated cocultures (CTRL) and CFBE cells cultured on Transwells were also analysed. Data were obtained from three experiments, each carried out in quadruplicate. Results are shown as mean ± SEM. ; . (b) Paracellular permeability (pAPP; cm/s) was measured after 6 days in the same experimental conditions as in (a). Data were obtained from three experiments, each carried out in triplicate. Results are shown as mean ± SEM. . (c) Representative dot plot showing viable (7-AAD(−) and annexin V(−)), necrotic (7-AAD(+) and annexin V(+)), early apoptotic (7-AAD(−) and annexin V(+)), and late apoptotic (7-AAD(+) and annexin V(−)) cells in all conditions as in (a). One representative experiment is shown.

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