Research Article
NADPH Oxidase Signaling Pathway Mediates Mesenchymal Stem Cell-Induced Inhibition of Hepatic Stellate Cell Activation
Figure 7
hBM-MSCs decrease the levels of 4-HNE and p47phox in HSCs in a mouse model of CCl4-induced liver fibrosis. As described in Figure 6, male mice were randomly separated into three groups; the mice of group 1 served as normal control group, and the other two groups of mice, respectively, received hBM-MSCs (8 × 106/mouse) or vehicle throughout the 11-week period of CCI4 (5 μl/g body weight, two times a week). (a) Double-staining was performed on liver section: synaptophysin (SYP, a marker for quiescent and activated HSCs) (green fluorescence); 4-HNE and p47phox (markers for positive HSCs) (red fluorescence); nuclei (blue fluorescence). The images were captured with the fluorescence microscope. Scale bar = 50 μm. The total HSCs (SYP-positive HSCs) and the relative number of 4-HNE and p47phox-positive HSCs in six randomly chosen fields were counted at 100-fold magnification, and the average values were shown. versus the control group. versus the mice of group treatment with CCI4 plus vehicle. (b) The protein levels of 4-HNE and p47phox were examined by Western blot analysis after the HSCs were isolated from each group. versus the control group. versus the mice of group treatment with CCI4 plus vehicle.
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