Lineage-Specific Chiral Biases of Human Embryonic Stem Cells during Differentiation

Figure 1

Human embryonic stem cell line, RUES2, displays no chiral bias. (a) Representative images of RUES2 cells expressing the nuclear marker DAPI (blue) and pluripotency markers Oct4, Nanog, and SOX2 (red). Over 95% of RUES2 cells exhibit Oct4, Nanog, and SOX2 expression. (b) A schematic showing planar rotation in the x-y plane about the z-axis. (c) RUES2 cells do not show a definitive chiral bias in rotation, displaying an almost 50 : 50 ratio of CW to CCW rotating cells. (d) A statistically significant number of cells rotated in plane compared to the other categories. (e) Quantitative data showing the number of cells in each category along with the number of replicate experiments (). The values indicate comparisons between CW and CCW and between planar rotation and the other three categories. Scale bars: 100 μm; .

(a)

(b)

(c)

(d)

(e)