Lineage-Specific Chiral Biases of Human Embryonic Stem Cells during Differentiation

Figure 2

Cardiac mesoderm cells display dominant CW biased rotation similar to a mouse cardiac cell line, HL-1. (a) Day 1 mesoendoderm cells expressing brachyury. (b) Day 5 cardiac mesoderm cells expressing Nkx2.5 (c) Mesoendoderm (D1-ME) shows no rotational bias, and cardiac mesoderm (D5-C.Meso) displays a CW bias along with the mouse cardiac muscle cell line, HL-1. (d) Mesoendoderm has more migrating, no-rotation, and complex rotating cells than planar rotating cells. Cardiac mesoderm shows no difference between the two sets of groups while HL-1 cells are primarily planar rotating. (e) Quantitative data showing the number of cells in each category along with the number of replicate experiments (). The values indicate comparisons between CW and CCW and between planar rotation and the other three categories. Scale bars: 100 μm; .

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