3D Culture of Bone Marrow-Derived Mesenchymal Stem Cells (BMSCs) Could Improve Bone Regeneration in 3D-Printed Porous Ti6Al4V Scaffolds

Table 1

Primer pair sequences for the studied genes.

Gene	Sequences		Annealing temperature (°C)
	Forward (5-3)	Reverse (3-5)
GAPDH	GTCAAGGCTGAGAACGGGAA	AAATGAGCCCCAGCCTTCTC	58
COL-1	CCTCGCTTTCCTTCCTCTCC	GTCAAGGCTGAGAACGGGAA	58
Runx2	CATGTCCCTCGGTATGTCCG	ACTCTGGCTTTGGGAAGAGC	58
OPN	ATCTCCTAGCCCCACAGACCC	CACACTATCACCTCGGCCATC	58
ALP	TCCTGTTGACACCCCAAACC	GGAAACGCAGGATTTCCCAC	58
OCN	CTCACACTCCTCGCCCTATTG	CGCCTGGGTCTCTTCACTAC	58