Location, Isolation, and Identification of Mesenchymal Stem Cells from Adult Human Sweat Glands

Figure 1

Histomorphology, immunocytochemical analysis, and ultrastructure of ahSGs in vivo. (a) Diagram showing each portion of ahSGs, which includes the intraepidermal, intradermal, and intraglandular duct and secretory portion. (b) H&E staining of the adult human skin (full thickness). The boxed area was magnified to determine that the solenoid bulb consisted of a duct and secretory portion. (c, d) Double immunofluorescence using the antibody combinations CEA or CK15 and α-SMA. The ahSG duct portion was positive for CEA and negative for CK15 (red) and α-SMA (green), whereas the secretory portion expressed CK15 (red) and α-SMA (green) with cell nuclei stained by Hoechst 33342 (blue). (e) TEM of the ahSG secretory portion. Myoepithelial cells were found in the outer portion of the glandular epithelial cells, and the pyramidal epithelial cells and flattened myoepithelial cells were closely related to each other. The outermost layer of the ahSG secretory portion was abundant ECM. Nuclei of myoepithelial cells were rich in heterochromatin that was darker, on the edge of the nucleus and around the nucleolus. Nu: nucleus; My: myoepithelial cell; G: glandular epithelial cell; E: ECM. (f) Immunoelectron microscopy of the ahSG secretary portion. The boxed area is magnified to visualize the myoepithelial cells. In the cytoplasm of the myoepithelial cells, the dispersive black dots are colloidal gold particles connected to α-SMA (white arrows indicate the colloidal gold particles). (b). Bar: 500 μm and 50 μm. (c) 400x. (d) 600x. (e) Bar: 5 μm. (f) Bar: 2 μm.

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