The Hypoxia-Mimetic Agent Cobalt Chloride Differently Affects Human Mesenchymal Stem Cells in Their Chondrogenic Potential

Figure 2

Effect of CoCl₂ in the expression of HIF-1α. The MSCs were incubated with 100 μM of CoCl₂ in cell medium supplemented with 2% of FCS for 6 h, 12 h, 24 h, and 48 h. (a) HIF-1α expression in the DPMSCs; (b) HIF-1α expression in the UCMSCs; (c) HIF-1α expression in the ADMSCs; (d) densitometric analysis of western blot bands. Control samples consist in the MSCs cultured with cell medium supplemented with 10% FCS for 48 h. The value was normalized to each corresponding β-actin level and represented as a relative expression compared to the control sample. Each value is the mean ± SD of triplicate independent experiments. , as compared to control DPMSCs; , as compared to control UCMSCs; , as compared to control ADMSCs.

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