Derivation and Identification of Motor Neurons from Human Urine-Derived Induced Pluripotent Stem Cells

Figure 3

NEPs derived from urine-iPSCs and B-iPSCs express neural progenitor markers. (a) Immunocytochemical staining of neural progenitor markers. NEPs derived from all three iPSC lines, B-iPSC, UC005, and UE017, were positive for the neural precursor markers, SOX1, SOX2, and Nestin. Cell nuclei were counterstained with 4,6-diamidino-2-phenylindole (DAPI). The scale bar is 50 μm. (b) The ratio of positive labeling for SOX1 and SOX2 in NEPs after 7 days of induction. NEPs from UC005 expressed higher levels of neural precursor markers. The ratio of positive labeling (%) = (number of positive cells/total number) × 100%. The results from three independent experiments are presented as the means ± standard errors of the mean (SEM). and according to Student’s t-test.

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