Derivation and Identification of Motor Neurons from Human Urine-Derived Induced Pluripotent Stem Cells

Figure 4

(a) PCR analysis of the mRNA expression levels of neural cell markers at every differentiation stage. After 7 days of differentiation, both B-NEPs and urine-NEPs expressed the neural markers, Nestin and SOX2. After an additional 6 days of induction, cells derived from all three iPSC lines expressed the MNP markers, Olig2 and Pax6. As early as day 19 of induction, MNs from B-iPSCs and urine-iPSCs expressed the MN-specific marker, HB9. These mRNA expression results were consistent with the results of protein expression, as shown by immunofluorescence. (b) Stages of differentiation of MNs from iPSCs and markers commonly used for their characterization.

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