Derivation and Identification of Motor Neurons from Human Urine-Derived Induced Pluripotent Stem Cells

Figure 5

Immunocytochemical staining and ratios of positive labeling of the MNPs and MNs. (a) After 13 days of induction, cells derived from all three iPSC lines, UC005, UE017, and B-iPSC, were positive for the MNP markers, Olig2 and Pax6. Cell nuclei were counterstained with 4,6-diamidino-2-phenylindole (DAPI). The scale bar is 50 μm. (b) After 19 days of induction, the cells derived from all three iPSC lines, UC005, UE017, and B-iPSC, were positive for the MN markers, HB9 and Islet1. Cell nuclei were counterstained with 4,6-diamidino-2-phenylindole (DAPI). The scale bar is 50 μm. (c) Cells from the UC005 and B-iPSC lines expressed higher levels of the MNP markers than the cells from the UE017 line. (d) The ratios of positive labeling for HB9 and Islet1 in MNPs after 19 days of induction. The ratios of positive labeling for HB9 were 79%, 74%, and 81% in MNs derived from UC005, UE017, and B-iPSC, respectively, but the difference was not statistically significant. A total of 84% of MNs from B-iPSC expressed Islet1, which was significantly higher than that of MNs from UC005 and UE017. The ratio of positive labeling (%) = (number of positive cells/total number) × 100%. , , and according to Student’s t-test.

(a)

(b)

(c)

(d)