Derivation and Identification of Motor Neurons from Human Urine-Derived Induced Pluripotent Stem Cells

Figure 6

Mature and functional MNs were generated after 26 days of differentiation. (a) Immunocytochemical staining of mature MNs. Under inductive conditions for more than 26 days, cells derived from all three iPSC lines, B-iPS, UE017, and UC005, expressed the mature MN marker choline acetyltransferase (ChAT). They also expressed the neuronal marker TuJ1. Cell nuclei were counterstained with 4,6-diamidino-2-phenylindole (DAPI). (b) Cocultured muscle cells and MNs form neuromuscular junctions. Immunofluorescence staining shows α-bungarotoxin- (BTX-) positive sites overlaid with TuJ1 labeling in the UC005 MNs (A, B, C). Cell nuclei were counterstained with 4,6-diamidino-2-phenylindole (DAPI). The scale bar is 50 μm.

(a)

(b)