Derivation and Identification of Motor Neurons from Human Urine-Derived Induced Pluripotent Stem Cells

Table 1

Primers used for reverse transcription polymerase chain reaction (RT-PCR).

Gene	Primer sequence (5-3)	Annealing (°C)
Nestin	F AGAAACAGGGCCTACAGAGC; R GAGGGAAGTCTTGGAGCCAC	65°C
SOX2	F CCCCCGGCGGCAATAGCA; R TCGGCGCCGGGGAGATACAT	60°C
Olig2	F CCCTAAAGGTGCGGATGCTT; R CTGGATGCGATTTGAGGAGC	65°C
Pax6	F CGGAGTGAATCA GCTCGGTG; R CCGCTTATACTGGGCTATTTTGC	60°C
HB9	F AGCACCAGTTCAAGCTCAACA; R ACCAAATCTTCACCTGGGTCTC	65°C
GAPDH	F ACCACAGTCCATGCCATCAC; R TCCACCACC CTGTTGCTGTA	60°C