Mesenchymal Stem Cells Promote the Osteogenesis in Collagen-Induced Arthritic Mice through the Inhibition of TNF-α

Figure 3

UC-MSC treatment significantly improved the impaired osteogenic differentiation ability in vitro. (a) Representative images showing ALP staining in intact (DBA/1), the CIA groups with no treatment, UC-MSC treated, anti-TNF-α, and zoledronic acid (ZA) treatment (photographed at ×100). (b) ALP activity was significantly deduced in the CIA group, and UC-MSC transplantation significantly increased ALP activity ( in each group). (c–e) UC-MSC transplantation significantly upregulated the mRNA levels of osteogenic genes including ALP, Osterix, and COL-I. (f–i) UC-MSC treatment significantly upregulated the ALP and Osterix protein levels in CIA mice. ; ; .

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