Mesenchymal Stem Cells Promote the Osteogenesis in Collagen-Induced Arthritic Mice through the Inhibition of TNF-α

Figure 4

Improved osteogenic differentiation of CIA under UC-MSC treatment may be related to inhibition of TNF-α. (a) The serum TNF-α level was significantly decreased in the UC-MSC group ( in each group). (b) Representative images showing ALP staining in CIA without or with TNF-α treatment. (c) ALP activity was significantly decreased in the TNF-α addition group. (d–i) The expression levels of osteogenic genes and protein levels of ALP and Osterix were significantly downregulated in the TNF-α addition group ( in each group). .

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