A Nonenzymatic and Automated Closed-Cycle Process for the Isolation of Mesenchymal Stromal Cells in Drug Delivery Applications

Figure 4

DB-ASC osteo-/adipo-differentiation. Osteogenic differentiation evaluated in Petri dish by Alizarin Red S staining (left) and by phosphatase alkaline staining (right). (a, c) DB-ASCs grown in control medium without supplements (negative control, CTRL−); (b, d) positive culture (200x magnification). Adipogenic differentiation evaluated by Oil Red staining (presence of red cytoplasmic inclusions; 200x magnification). (e) Negative control (CTRL−) (200x magnification). (f) Positive culture (200x magnification).