Type III Transforming Growth Factor-<i>β</i> Receptor RNA Interference Enhances Transforming Growth Factor <i>β</i>3-Induced Chondrogenesis Signaling in Human Mesenchymal Stem Cells

<div>SB431542 blocked T<i>β</i>RIII RNAi-activated TGF-<i>β</i>3-mediated phosphorylation of Smad2/3. SiNC- or SiT<i>β</i>RIII-infected hMSCs were cultured in chondrogenic medium, supplemented with TGF-<i>β</i>3 (T-SiNC group or T-SiT<i>β</i>RIII group) and exposed to SB431542 treatment for 2 h before treatment with TGF-<i>β</i>3 (T-SiNC + SB group or T-SiT<i>β</i>RIII + SB group). Samples were harvested after 24 h. (a) Western blot of protein levels of P-Smad2/3, total Smad2/3, and GAPDH. (b) Quantification of protein levels of P-Smad2/3 normalized to total levels of Smad2/3 and GAPDH. Error bars represent the means ± SD, <span class="nowrap"><svg height="8.55521pt" id="M64" style="vertical-align:-0.2063904pt" version="1.1" viewbox="-0.0498162 -8.34882 27.8368 8.55521" width="27.8368pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M495 86L479 114C446 82 419 66 409 66C401 66 401 72 406 97C420 166 436 231 453 297C489 435 454 448 428 448C406 448 384 439 354 422C305 394 222 327 161 247H159L183 345C200 415 194 448 173 448C143 448 82 410 23 351L38 325C64 349 95 371 105 371C111 371 116 365 109 336L25 -4L31 -12C50 -4 77 3 107 9C119 69 132 122 145 168C197 254 321 381 370 381C387 381 393 374 378 305L329 95C309 17 320 -12 345 -12C372 -12 430 19 495 86Z" id="g113-111"></path></g><g transform="matrix(.013,0,0,-0.013,10.158,0)"><path d="M535 323V373H52V323H535ZM535 138V188H52V138H535Z" id="g117-34"></path></g><g transform="matrix(.013,0,0,-0.013,21.421,0)"><path d="M285 378C315 398 338 416 353 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80 96 71 90C59 80 50 67 49 57C48 45 52 36 66 23C80 9 123 -12 169 -12C221 -11 288 15 342 59C403 109 431 165 431 225C431 308 366 395 238 395C212 395 165 379 127 364L153 550Z" id="g113-54"></path></g></svg> versus T-SiT<i>β</i>RIII group.</div>

Stem Cells International

fig6

Figure 6

Figure 6: Type III Transforming Growth Factor-<i>β</i> Receptor RNA Interference Enhances Transforming Growth Factor <i>β</i>3-Induced Chondrogenesis Signaling in Human Mesenchymal Stem Cells 

Figure 6 | Type III Transforming Growth Factor-β Receptor RNA Interference Enhances Transforming Growth Factor β3-Induced Chondrogenesis Signaling in Human Mesenchymal Stem Cells 