Osteogenic Potential of Human Umbilical Cord Mesenchymal Stem Cells on Coralline Hydroxyapatite/Calcium Carbonate Microparticles

Figure 3

SEM images of organoids with UCM (A) and BM (B) MSCs during the process of osteogenesis. Images were taken on days 1 (A1, B1), 7 (A2, B2), 14 (A3, B3), and 21 (A4, B4) of the differentiation process and compared to a control (α-MEM) image at day 21 (A5, B5). Over time, the voids within the organoid were filled with MSCs and associated extracellular matrix which formed a smoother surface that covered CHACC surfaces. Significant coverage was exhibited using BM MSCs at day 14 (B3), whereas UCM MSCs did not cover surfaces until day 21 (A4). Scale bars: 500 μm.