Differentiation of Human Embryonic Stem Cells to Sympathetic Neurons: A Potential Model for Understanding Neuroblastoma Pathogenesis

<div>H9 hESC live cell staining for pluripotency markers SSEA4 and TRA-1-60. (a-i, b-i) Phase contrast microscopy. (a-ii, b-ii) Hoechst staining showing efflux of Hoechst from stem cells but not feeder layer. (a-iii) SSEA-4 (red) and (b-iii) TRA-1-60 (green) showing specific staining for human ES cell colonies compared with control feeder cells. White arrows highlight the stem cell colony borders.</div>

Stem Cells International

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Figure 2: Differentiation of Human Embryonic Stem Cells to Sympathetic Neurons: A Potential Model for Understanding Neuroblastoma Pathogenesis 