Research Article
Differentiation of Human Embryonic Stem Cells to Sympathetic Neurons: A Potential Model for Understanding Neuroblastoma Pathogenesis
Table 1
Comparison of the current study with previously published studies reporting the differentiation of pluripotent stem cells to sympathetic neurons.
| Reference | Methods | Cell type | Markers used to identify SNS progenitors | Yield |
| Mizuseki et al. [9] | PA6 stromal cells/BMP4 | mESCs, primate ESCs | TH+/PRPH+/Phox2b | Not quantified—very low | Pomp et al. [33] | PA6 coculture BHK-21 medium/Glasgow MEM + N2 supplement | HES1, HUES1, HUES7 | TH+/PRPH+ | <1% | Lee et al. [25] | MS5 stromal cell line 28 days, FGF2/EGF exposure | H9 | TH+/PRPH+ | 1-2% | Jiang et al. [24] | PA6 stromal cell line—7 days BHK-21 medium/Glasgow modified Eagle’s medium + N2 supplement | H1, H9 | TH+/PRPH+ | 18% | Acevedo et al. [34] | Embryoid bodies (EBs)—onto collagen plates | H9 | Dopa decarboxylase (DDC), TH+, Mash 1 | Not determined | Huang et al. [10] | Exposure to retinoic acid, BMP2, BMP4, BMP7 | H1, WTC iPSC | TH+/DBH+ | Not determined | This study | PA6 stromal cells, MACS® neuronal media + BMP4 | H9 | TH+/PRPH+, DBH+/PHOX2B+ | 20% and 9.4% ± 5.5%, respectively |
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