Differentiation of Human Embryonic Stem Cells to Sympathetic Neurons: A Potential Model for Understanding Neuroblastoma Pathogenesis

Table 1

Comparison of the current study with previously published studies reporting the differentiation of pluripotent stem cells to sympathetic neurons.

Reference	Methods	Cell type	Markers used to identify SNS progenitors	Yield
Mizuseki et al. [9]	PA6 stromal cells/BMP4	mESCs, primate ESCs	TH+/PRPH+/Phox2b	Not quantified—very low
Pomp et al. [33]	PA6 coculture BHK-21 medium/Glasgow MEM + N2 supplement	HES1, HUES1, HUES7	TH+/PRPH+	<1%
Lee et al. [25]	MS5 stromal cell line 28 days, FGF2/EGF exposure	H9	TH+/PRPH+	1-2%
Jiang et al. [24]	PA6 stromal cell line—7 days; BHK-21 medium/Glasgow modified Eagle’s medium + N2 supplement	H1, H9	TH+/PRPH+	18%
Acevedo et al. [34]	Embryoid bodies (EBs)—onto collagen plates	H9	Dopa decarboxylase (DDC), TH+, Mash 1	Not determined
Huang et al. [10]	Exposure to retinoic acid, BMP2, BMP4, BMP7	H1, WTC iPSC	TH+/DBH+	Not determined
This study	PA6 stromal cells, MACS® neuronal media + BMP4	H9	TH+/PRPH+, DBH+/PHOX2B+	20% and 9.4% ± 5.5%, respectively