Research Article

Differentiation of Human Embryonic Stem Cells to Sympathetic Neurons: A Potential Model for Understanding Neuroblastoma Pathogenesis

Table 1

Comparison of the current study with previously published studies reporting the differentiation of pluripotent stem cells to sympathetic neurons.

ReferenceMethodsCell typeMarkers used to identify SNS progenitorsYield

Mizuseki et al. [9]PA6 stromal cells/BMP4mESCs, primate ESCsTH+/PRPH+/Phox2bNot quantified—very low
Pomp et al. [33]PA6 coculture BHK-21 medium/Glasgow MEM + N2 supplementHES1, HUES1, HUES7TH+/PRPH+<1%
Lee et al. [25]MS5 stromal cell line 28 days, FGF2/EGF exposureH9TH+/PRPH+1-2%
Jiang et al. [24]PA6 stromal cell line—7 days
BHK-21 medium/Glasgow modified Eagle’s medium + N2 supplement
H1, H9TH+/PRPH+18%
Acevedo et al. [34]Embryoid bodies (EBs)—onto collagen platesH9Dopa decarboxylase (DDC), TH+, Mash 1Not determined
Huang et al. [10]Exposure to retinoic acid, BMP2, BMP4, BMP7H1, WTC iPSCTH+/DBH+Not determined
This studyPA6 stromal cells, MACS® neuronal media + BMP4H9TH+/PRPH+, DBH+/PHOX2B+20% and 9.4% ± 5.5%, respectively