Synergistic Integration of Mesenchymal Stem Cells and Hydrostatic Pressure in the Expansion and Maintenance of Human Hematopoietic/Progenitor Cells

Figure 3

Flow cytometry analysis of expanded cells under different culture conditions. Proportion of cells expressing the typical HSPC marker (a) CD34⁺ and primitive HSPC markers (c) CD34⁺CD38⁻ and (e) CD133⁺CD38⁻ according to coculture with MSCs and adherence. Proportion of cells expressing (b) CD34⁺, (d) CD34⁺CD38⁻, and (f) CD133⁺CD38⁻ after applying hydrostatic pressure (HP) on days 3 and 4, (, ).

(a)

(b)

(c)

(d)

(e)

(f)