Neural Stem Cell-Conditioned Medium Ameliorated Cerebral Ischemia-Reperfusion Injury in Rats

Figure 2

The effect of NSC-CM on cell apoptosis and mitochondrial ultrastructure in the ischemic sphere. Representative TUNEL staining image on the 3rd day after cerebral ischemia onset of the control group (a) and the NSC-CM group (b). The brown particle is the TUNEL-positive cells whereas the blue particle is the normal cells (c). The protein expression of Bcl-2 in the cerebral ischemia penumbra of 2 groups assessed by Western blot. Control: the group receiving PBS by tail vein injection; NSC-CM: the group receiving NSC-CM by tail vein injection. Representative mitochondrial ultrastructures of the control group (d) and the NSC-CM group (e) in neuron. The black arrows indicated swollen mitochondria with disintegrated or disappeared cristase. The red arrows indicated normal mitochondria with integrated cristase. Bar: 500 nm (f). The bar graph showing that NSC-CM significantly decreased the number of TUNEL-positive cells in cerebral ischemic hemisphere, compared to the control group. , two-sample t test (g). The bar graph showing that NSC-CM significantly increased the expression of Bcl-2 in cerebral ischemic hemisphere, compared to control group. , Two-sample t test.

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