Developmental Pathways Pervade Stem Cell Responses to Evolving Extracellular Matrices of 3D Bioprinted Microenvironments

Figure 4

GO analysis of hMSCs in 3D bioprinted prisms, especially related to the differentiation state. (a) Counts of GO terms associated with commonly examined stem cell responses. Protein degradation, development, and differentiation are the most significantly expressed pathways. (b) Development and differentiation pathways separated by 3D ECM structures. Most influential 3D structures rank from 3D/LN > 3D/Col1 > 3D/BSA > 3D/FN, with 3D/FN structures predominantly influencing vasculature development. Expression of endothelial cell markers (c), smooth muscle cell markers (d), and sphingosine phosphate lyase (e) in hMSCs cultured in 3D/FN structures. Error bars depict SD associated with experimental replicates with at least 2 different prisms analyzed per experimental replicate. hMSCs cultured in 3D/FN structures expressed markers for both endothelial and smooth muscle cells and had a reduction in SGPL1 expression.

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