Generation of Retinal Organoids with Mature Rods and Cones from Urine-Derived Human Induced Pluripotent Stem Cells

<div>Characteristics of urine-hiPSCs. (a) The schematic of reprogramming process of integration-free hiPSCs from urine cells. (b) Representative phase contrast photograph of U-hiPSCs. Scale bar = 250 <i>μ</i>m. (c) RT-PCR showed that U-hiPSCs from all three lines expressed pluripotency markers OCT4 and SOX2. noRT: negative control without reserve transcriptase. (d) Example images of immunofluorescence staining of U-hiPSCs with pluripotency markers OCT4, NANOG, SSEA4, TRA-1-81, and TRA-1-60. Scale bars = 20 <i>μ</i>m.</div>

Stem Cells International

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Figure 1

Figure 1: Generation of Retinal Organoids with Mature Rods and Cones from Urine-Derived Human Induced Pluripotent Stem Cells 