Osteogenic Effect and Cell Signaling Activation of Extremely Low-Frequency Pulsed Electromagnetic Fields in Adipose-Derived Mesenchymal Stromal Cells

Table 2

Forward and reverse primer sequences and annealing temperature for the respective genes.

Gene	Forward primer	Reverse primer	Annealing temperature (°C)
RunX2	TGCCTAGGCGCATTTCAGGTGC	TGAGGTGATGGCGGGGTGT	60
Osteocalcin	CCAGCGGTGCAGAGTCCAGC	GACACCCTAGACCGGGCCGT	60
Osteopontin	CTCCATTGACTCGAACGACTC	CGTCTGTAGCATCAGGGTACTG	60
Alkaline phosphatase	ACGTGGCTAAGAATGTCATC	CTGGTAGGCGATGTCCTTA	57.5
Collagen I	AGCGGACGCTAACCCCCTCC	CAGACGGGACAGCACTCGCC	60
Βeta-tubulin	GAGGGCGAGGACGAGGCTTA	TCTAACAGAGGCAAAACTGAGCACC	60