Radiation Induces Apoptosis and Osteogenic Impairment through miR-22-Mediated Intracellular Oxidative Stress in Bone Marrow Mesenchymal Stem Cells

Figure 3

Cellular apoptosis and ROS generation in miR-22-modified rBMSCs following 6 Gy IR. After transfection of miR-22 lentivirus with 48 h, cells were than exposed to IR and samples were collected at 24 h postradiation. (a) Overexpression of miR-22 enhanced the apoptotic ratio of rBMSCs (). (b–d) Inhibition of miR-22 helps cells against the production of ROS. (b) ROS staining. (c, d) Mean fluorescent intensity and the ROS-positive ratio (%) (, and ). (e) Protein level of SOD2, NOX4, and Caspase-3 in miR-22-modified cells following IR. (f) The densitometric intensity analysis. Both showed that miR-22 played an important role on IR-induced ROS generation and apoptosis, vice versa. All bar graphs were shown as (, , , and ).

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