Alteration in the Cytokine Secretion of Bone Marrow Stromal Cells from Patients with Chronic Myelomonocytic Leukemia Contribute to Impaired Hematopoietic Supportive Activity

Figure 2

Analysis of cells in the upper chamber after a 3-day transwell coculture of CB CD34⁺ cells with HD-BMSCs or CMML-BMSCs. (a) Quantitation of cell numbers in the upper chamber after a 3-day coculture. (b) Flow cytometric analysis of CD34⁺ cell populations in the upper chamber after a 3-day coculture of CB CD34⁺ cells with HD-BMSCs or CMML-BMSCs. Quantification of the percentage of CD34⁺ cell population in the upper chamber is shown in the bar graph. (c) Flow cytometric analysis of CMP, GMP, and MEP populations in the upper chamber after a 3-day coculture of CB CD34⁺ cells with HD-BMSCs or CMML-BMSCs. Quantification of the percentage of CMP, GMP, and MEP populations in the upper chamber is shown in the bar graph (). Data are presented as the , ; ns: not significant.

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