Guiding the Differentiation Direction of Pancreatic Islet-Derived Stem Cells by Glycated Collagen

Figure 1

The cytoskeleton structure of PI-MSCs cultured on Col (b), Col-G (c), Col-M (d), Col-A (e), and Col-R (f) was shown after the staining with FITC-phalloidin (scale bars: 50 μm). Uncoated glass surface (a) was used as a control. The cell attachment on the collagen surfaces was given as the percentage with respect to the unmodified Col (g). Only cells on the rhamnose-modified collagen showed a significant decrease in the cell attachment (). The alteration of the proliferation rates after the culture on the collagens was estimated by WST-1 assay (h). All the surfaces coated with modified collagens supported the cell proliferation higher than the unmodified collagen ().

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