Recovery of Donor Hematopoiesis after Graft Failure and Second Hematopoietic Stem Cell Transplantation with Intraosseous Administration of Mesenchymal Stromal Cells

Table 4

Proportion of donor cells in the bone marrow (BM), CFU-Fs, and MSCs.

Proportion of donor chimerism, %
Time after MSC injection directly into the trabecular bone area
Patients	1-2 months			4-5 months			9 months
	BM	CFU-F	MSCs	BM	CFU-F	MSCs	BM	CFU-F	MSCs
1 M	25–50	ND	6.9 ± 0.5	100	78 ± 4	3.2 ± 2.2	100	62 ± 5	7.2 ± 0.6
2 P	100	ND	5.0 ± 0.9	Death on the 68th day
3 CH	100	65.5 ± 19.7	2.0 ± 0.8	100	57 ± 6	21.7 ± 2.0	100	100	7.3 ± 2.4
4 F	100	ND	3.1 ± 0.002	Death on the 63rd day
7 SA	100	79.5 ± 12.1	22.3 ± 4.6	ND	ND	ND	ND	ND	ND
8 CHA	100	50.0 ± 2.0	9.3 ± 4.2	ND	ND	ND	ND	ND	ND

For each patient, MSCs were obtained from the bone marrow taken from 2–4 points of the iliac bones. The data are presented as the average percentage of donor MSCs at 1–3 passages from all investigated bone marrow locations ± standard error. ND: no data. The sensitivity limit of method used was 1%.