High Throughput Screening of Additives Using Factorial Design to Promote Survival of Stored Cultured Epithelial Sheets

Table 1

Tested additives and suppliers.

Additive	Concentration	Supplier
Acetovanillone	50 μM	Sigma-Aldrich (St. Louis, MO)
Adenosine	5 mM	Sigma-Aldrich (St. Louis, MO)
Allopurinol	1 mM	Sigma-Aldrich (St. Louis, MO)
Antimycin A	20 nM	Sigma-Aldrich (St. Louis, MO)
Aspirin (acetylsalicylic acid)	133–400 μg/ml	Sigma-Aldrich (St. Louis, MO)
ATP (adenosine triphosphate)	67–200 μM	Sigma-Aldrich (St. Louis, MO)
Dimethyl (S)-(−)-malate	2,5 mM	Sigma-Aldrich (St. Louis, MO)
DMSO (dimethyl sulfoxide)	0.07–0.20%	Sigma-Aldrich (St. Louis, MO)
Fenoldopam mesylate	6.7–20 μM	Sigma-Aldrich (St. Louis, MO)
L-Gluthatione	3 mM	Sigma-Aldrich (St. Louis, MO)
Glycerol	7.5–100 mg/ml	Sigma-Aldrich (St. Louis, MO)
Hydrocortizone	3 ng/ml	Sigma-Aldrich (St. Louis, MO)
Icilin	0.33–1 μM	Santa Cruz Biotechnology (Heidelberg, Germany)
Insulin	5 μg/ml	Sigma-Aldrich (St. Louis, MO)
Lactic acid	13–40 mM	Sigma-Aldrich (St. Louis, MO)
L-Ascorbic acid	50 μg/ml	Sigma-Aldrich (St. Louis, MO)
L-carnosine	13.3–40 mM	Sigma-Aldrich (St. Louis, MO)
Leukemia inhibitory factor (LIF)	13–40 ng/ml	Sigma-Aldrich (St. Louis, MO)
LiCl	1 mM	Merck (Oslo, Norway)
Melatonin	0.67–2 nM	Calbiochem (San Diego, CA)
Menthol	16.7–50 μM	Santa Cruz Biotechnology (Heidelberg, Germany).
Methyl pyruvate	1 mM	Sigma-Aldrich (St. Louis, MO)
N-(2-Mercaptopropionyl)glycine (NMPG)	100 μM	Sigma-Aldrich (St. Louis, MO)
N-Acetyl-L-cysteine	1 mM	Sigma-Aldrich (St. Louis, MO)
Sodium pyruvate	10 mM	Sigma-Aldrich (St. Louis, MO)
Taurine	20 mM	Sigma-Aldrich (St. Louis, MO)