High Throughput Screening of Additives Using Factorial Design to Promote Survival of Stored Cultured Epithelial Sheets

Table 2

Summary of experiments.

Exp. #	Design type	Donor/source	Storage time	Tested additives
1A; Storage in MEM	2^10	♀age 53, abdomen	7 days	Glycerol (1%)
				L-Ascorbic acid
				Allopurinol
				Sodium pyruvate
				Adenosine
				Taurine
1B; Storage in CnT Prime				L-Glutathione
				Hydrocortizone
				LiCl
				Antimycin-A
2	2^10	♀age 40, abdomen	7 days	Glycerol 0.75%
				Glycerol 3%
				Icilin
				Menthol
				Dimethyl (S)-(−)-malate
				Methyl pyruvate
				N-Acetyl-L-cysteine
				Insulin
				Acetovanillone
				N-(2-Mercaptopropionyl)glycine (NMPG)
3	5^5	♀ age 47, breast	11 days	L-carnosine
				DMSO
				Fenoldopam mesylate
				Glycerol (up to 6%)
				LIF
4	5^5	♀age 40, abdomen	11days	Glycerol (up to 10%)
				Aspirin
				Melatonin
				Lactic acid
				ATP