MicroRNA-1 Regulates the Differentiation of Adipose-Derived Stem Cells into Cardiomyocyte-Like Cells

Figure 6

Immunofluorescence detection of the myocardium-specific transcription factor GATA in ASCs overexpressing miRNA-1 and cocultured with CMs. Five groups of 3rd-generation lentivirus (Lv)-infected ASCs and/or CMs were examined as follows: (a–d) CMs alone; (e–h) Lv-miR-1-CMs (miRNA-1-expressing ASCs cocultured with CMs); (i–l) Lv-NC-CMs (control infected ASCs cocultured with CMs); (m–p) Lv-miR-1 cells (miRNA-1-expressing ASCs); (q–t) ASCs alone. (a, e, i, m, and q) Merged images; (b, f, j, n, and r) DAPI fluorescence stain; (c, g, k, o, and s) GFP, indicating ASC fluorescence labeling; (d, h, l, p and t) red fluorescence, indicating GATA4.

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