Fish Scale-Derived Scaffolds for Culturing Human Corneal Endothelial Cells

Figure 6

Histological analysis using periodic acid-Schiff staining. (a) A control human cornea showing the presence of Descemet’s membrane and endothelial cells using PAS staining at 100x and (b) 400x. (c) FSS showing monolayer of endothelial cells at 200x magnification and (d) at 400x magnification. Histology did not show any clear evidence or development of Descemet’s membrane on FSS by the HCEnCs but showed a monolayer of cells attached in most of the areas. (e) Collagen VI and (f) laminin as the extracellular markers did not express on the cells at day 11. Nucleus can be seen in red marked with Draq 5. FSS: fish scale scaffold; HCEnC: human corneal endothelial cell.

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