The Effects of Conditioned Medium Derived from Mesenchymal Stem Cells Cocultured with Hepatocytes on Damaged Hepatocytes and Acute Liver Failure in Rats

Figure 1

Characterization of isolated MSCs and hepatocytes and cocultured MSCs and hepatocytes. (a) Phase-contrast micrographs of rat MSCs from passage 4 at day 3 of culture. (b) Expression of MSC markers. MSCs were positive for CD29 and negative for CD34 and CD45. (c) Primary hepatocytes exhibited compact and round morphology after 24 hours of monoculture. (d) Hepatocytes cocultured with MSCs displayed a polyhedral shape with well-demarcated cell-cell borders, distinct nuclei, and binucleate. Bar = 100 μm.

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