Intravenous Transplantation of Mesenchymal Stem Cells Reduces the Number of Infiltrated Ly6C⁺ Cells but Enhances the Proportions Positive for BDNF, TNF-1α, and IL-1β in the Infarct Cortices of dMCAO Rats

Figure 7

CD68⁺ microglia but not Iba-1⁺ cells express IL-β in the ischemic ipsilateral hemisphere at day 2. (a–d) Distribution of Iba-1⁺ cells and IL-β⁺ cells in the infarct areas after MSC transplantation. (e–h) Distribution of Iba-1⁺ cells and IL-β⁺ cells in the striatum after MSC transplantation. (i–l) Distribution of CD68⁺ cells and IL-β⁺ cells in the infarct areas after MSC transplantation. (a, e) Iba-1 staining. (i) CD68 staining. (b, f, j) IL-β. (c, g, k) DAPI nucleus staining. (d) No double-stained Iba-1⁺/IL-β⁺ cells were found in the infarct area after dMCAO. (h) No Iba-1⁺/IL-β⁺ cells were found 2 days after MSC transplantation in the striatum and corpus callosum after MSC transplantation. (i–l) Some CD68⁺ cells were positive for IL-β. Arrow: double-labeled cells. Scale bar, 250 μm.