Chemical Activation of the Hypoxia-Inducible Factor Reversibly Reduces Tendon Stem Cell Proliferation, Inhibits Their Differentiation, and Maintains Cell Undifferentiation

Figure 3

Effects of HIF-1α activation on hTSC morphology, proliferation, and viability. (a) Phase-contrast microphotographs (original magnification ×10), (b) cell growth curves, (c) MTT assay, (d) RealTime-Glo assay, (e) CellTox assay, and (f) Annexin V/PI staining of hTSCs during a 96 h treatment with different concentrations of DMOG (0.01 mM, 0.1 mM, and 1 mM) in normal growth medium and compared to control cells cultured without DMOG (0 mM). All experiments were performed in triplicates. Error bars show the mean ± SD of six and three different experiments in the case of cell growth curves and MTT assay, respectively. values were calculated using Student’s t-test. Only values < 0.05 are indicated: , , , and , as compared to control cells. Bar = 100 μm.

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