Human Bone Marrow Mesenchymal Stem Cells Promote Gastric Cancer Growth via Regulating c-Myc

Figure 4

hBM-MSC-CM promoted migration and glucose uptake of MGC-803 and BGC-823 cells. (a, c) Transwell migration assays showed that hBM-MSC-CM increased the migratory ability of gastric cancer cells and that this activity was inhibited by JQ1 (scale bar: 100 μm). (b, d) The numbers of migrated cells were analyzed by a one-way analysis of variance (, ). (e) c-Myc siRNA can inhibit the expression of c-Myc induced by hBM-MSC-CM in MGC-803 cells. (f, g) The effect of hBM-MSC-CM on MGC-803 cell migration can be suppressed by c-Myc siRNA (). (h) hBM-MSC-CM-induced Glut1 upregulation in MGC-803 cells was detected with Western blot. (i, j) The glucose uptake of MGC-803 and BGC-823 cells after the indicated treatments was detected using a clinical chemistry analyzer (, , ).

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