Paracrine Proangiogenic Function of Human Bone Marrow-Derived Mesenchymal Stem Cells Is Not Affected by Chronic Kidney Disease

Figure 2

Proliferation and senescence-associated- (SA-) β-galactosidase activity assay. (a) Representative xCELLigence Cell Index curve. (b) PDT = population doubling time. Proliferation of CKD MSCs did not differ from healthy MSCs (ratio CKD : healthy 0.79, 95% CI: 0.54-1.15, ). (c) Senescence-associated- (SA-) β-galactosidase activity assay. Representative example of C12FDG fluorescence as detected with a SA-β-galactosidase activity assay to detect senescence. The black line represents the negative control. (d) Senescence is higher in healthy MSCs when compared to CKD MSCs (ratio CKD : healthy 0.79, 95% CI: 0.64-0.99, ). (e) Linear regression analysis did not show an association between senescence and age (). (f) Senescence was higher in MSCs derived from female participants (ratio female : male 1.38, 95% CI: 1.12-1.69, ).

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