Paracrine Proangiogenic Function of Human Bone Marrow-Derived Mesenchymal Stem Cells Is Not Affected by Chronic Kidney Disease

Figure 3

Paracrine angiogenic function assays with MSC-derived serum-free conditioned medium (CM). (a) Scratch wound migration assay using MSC CM on HMEC-1 cells. Representative images of healthy MSC CM and CKD MSC CM at 0 and 6 hrs. (b) Quantification of scratch wound closure. Delta scratch width was normalized to the positive control. There was no statistically significant difference between CKD and healthy MSC CM (ratio CKD : healthy 1.28, 95% CI: 0.89 to 1.77, ). (c) Matrigel angiogenesis assay using MSC CM on HMEC-1 cells. Representative images of healthy and CKD MSC CM. (d) Quantification of tubule formation, defined as junction/tubule length. The tubule length/junction ratio was normalized to the positive control for each participant. There was no statistically significant difference between CKD and healthy MSC CM (ratio CKD : healthy 0.89, 95% CI: 0.76-1.05, ).

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